Rapigest price Contact a supplier or the parent company directly to get a quote or to find out a price or your closest point of sale. To test the efficacy of Rapigest for proteomics analysis of different sample types we used protein extracts from S9 cell line and mouse heart tissue PAGE 1 [ Care and Use Manual ] RapiGest SF Surfactant I. This basic kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, and the GlycoWorks Clean-up Module (excludes PN 186007988). 5:1), and an incubation time of 2 h. 2% Rapigest in final concentration pre-digestion. Digestion parameters TIP: The surfactant RapiGest SF breaks-down into non-interfering and non-ion suppressing products by acidifying final sample mix. You can also find out where to buy gold coins from gold dealers at the The mass spectrometry-compatible surfactant RapiGest promotes the enzymatic digestion of proteins by facilitating their unfolding while retaining enzymatic activity. Add RapiGest™ SF to your proteolytic digestion, (using trypsin or other enzymes) to enhance digestion speed and increase peptide recovery. ANSWER: 0. Mar 6, 2025 · Waters RapiGest SF Surfactant 10 mg Life Sciences Trading (1511) 99. 035 mL/vial Rapid PNGaseF Enzyme (1) 0. 5% (w/v)What is the best RapiGest SF concentration to use in cell lysis with a denaturing protocol? - WKB194441 Last updated Save as PDF Share Share Tweet Share Article number: 194441 ENVIRONMENT ANSWER ADDITIONAL INFORMATION ANSWER: The digestion time is approximately one hour. View specifications, prices, citations, reviews, and more. Flex and Focus custom panels, tissue GOLDPRICE. 3 (A) Comparison of the fresh and FFPE tissues extracted with RapiGest and FASP protocols (B) Comparison of protocol efficiency over fresh and FFPE tissues. The digestion speed and more peptide recovery is enhanced by using trypsin or other enzymes. US Patent No: 8,580,533 RapiGest™ SF is available in a variety of formats. Mild protein denaturation opens the protein structure and exposes the proteolytic sites to enzymatic cleavage. <br> RapiGest™ SF is available in a variety of formats. Contact Us. <br><br> RapiGest SF is available in a variety of formats. 00/ea Sep 17, 2010 · 结论 • RapiGest SF 促进了蛋白酶解的速度与完全程度，能够得到蛋白药物序列覆盖率很高的肽图分析。 • RapiGest SF 是适用于蛋白组学、糖蛋白与生物制药应用的领域 • 几乎无需消解后样品处理，简单样品酸化，足以从溶液中去除 RapiGest SF 。 Protein extraction with a combination of GnHCl followed by RapiGest™ on the insoluble pellet demonstrated, after label-free quantification, increased abundance of identified collagen proteins and low sample to sample variability. Compare Proteomics Services from leading suppliers on Biocompare. RapiGest SF, 10 mg, 4/pk Enjoy exclusive benefits including discounted pricing on orders by contacting our Sales Executives to open an account. Sep 11, 2025 · Rapigest SF can be a vital tool for efficient extraction and clean-up. *Note: If you use 50-100mM Tris pH 8. This part contains one, 50 mg vial for large scale digestions. Contact Us View and Download Waters RapiGest SF Surfactant instruction manual online. Contact Us SF Protein Digestion Surfactant Waters patented* RapiGestTM SF (surfactant) radically enhances protein enzymatic digestions in terms of speed and percent recovery. 7% positive Seller's other items Contact seller US $270. 1-0. b. RapiGest SF RapiGest SF, the brand-name for sodium 3- [ (2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate, is an acid-cleavable anionic detergent marketed by Waters Corporation and AOBIOUS INC. , excl. RapiGest SF, 50 mg, 1/pk Originally created as an enzyme-friendly surfactant meant to assist in-solution protein digestion, RapiGest SF is an innovative evolution of the product that helps to improve protein solubility during preparation of samples. RapiGest SF is available in a variety of formats. UChicago Proteomics Platform will purchase it and charge it at market prices to the customers. at pH 2 and 60 min. *Olink Proteomics pricing includes all consumables, reagents, and sequencing costs. 5, add twice as much HCl prior to MS analysis. We provide you with timely and accurate silver and gold price commentary, gold price history charts for the past 1 days, 3 days, 30 days, 60 days, 1, 2, 5, 10, 15, 20, 30 and up to 43 years. The kit contains: GlycoWorks Deglycosylation Module: (4) 0. Has anyone prepard 帮助蛋白质消化的强大工具 RapiGest SF（表面活性剂）可根本性地提高蛋白酶解的速度和回收率。RapiGest SF是一种温和的变性剂，有助于溶解和展开蛋白质，从而使其更易于被裂解，同时又不变性或抑制常规蛋白酶的活性。 不同于SDS或尿素等常见变性剂，RapiGest SF并不会修饰多肽或抑制常规蛋白酶活性 Waters Delivery Time approx. The RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. GnHCl and Rapigest combined protein extraction 450 μL GnHCl and RapiGest™ extraction buffer (0. S POOL samples representative proteomes for each tissue type and protocol were selected based on proteins identified in at least two out of three technical replicates in MS E and UDMS E acquisition modes, respectively. This part contains five, 1mg RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. The digestion method employs RapiGest SF (an acid-labile surfactant), an excess amount of modified trypsin (enzyme-to-substrate ratio of 2. This part contains 4, 10 mg vials Mar 6, 2025 · Waters RapiGest SF Surfactant 10 mg Life Sciences Trading (1511) 99. Shipping Qty Add to Cart Add to Compare Details: Waters RapiGest SF, 50 mg, 1/pk; Skip to the end of the images gallery Skip to the beginning of the images gallery More Information More Information Search Terms Waters SF Protein Digestion Surfactant Waters patented* RapiGestTM SF (surfactant) radically enhances protein enzymatic digestions in terms of speed and percent recovery. 3. reconStItutIon of rapIgeSt Sf powder Shown in Table u0018, are the volumes required to reconstitute the u0018 mg Rapi Gest SF powder in water or buffer to obtain preferred concentra- tions. In contrast, GnHCl extraction on its own showed increased abundance of identified proteoglycans and cellular proteins. Add 100 μl 0. Close and edit product RapiGest SF 10mg 1/pkg Price: $510. It depends heavily on several things. 1. Research our lab equipment offerings, including HPLC, UHPLC, UPLC, and MS Systems, and shop with us today! Google Finance provides real-time market quotes, international exchanges, up-to-date financial news, and analytics to help you make more informed trading and investment decisions. Oct 17, 2025 · Track the current silver spot price and market trends at APMEX, your trusted source for precious metals pricing and investment insights. More complex denaturation of globular proteins can be accomplished by heating in a Rapi Gest SF solution at elevated temperatures We would like to show you a description here but the site won’t allow us. RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. Unlike other commonly used denaturants, such as SDS or urea, RapiGest SF does not modify peptides or suppress protease activity RapiGest SF Surfactant Protocol Use the RapiGest SF Surfactant from RapiGest Surfactant from Waters to extract and solubilize hydrophobic proteins and improve in-solution enzymatic digestions of proteins. We present a novel objective In order to maximize solubility of proteins, add calculated volume of Waters Rapigest to have 0. When samples are acidified after tryptic digestio it breaks down into by-products that can be removed / are compatible with downstream MS analysis. com: Luster Leaf 1840 Rapitest Soil pH Meter : Patio, Lawn & GardenThe #1840 Soil pH Meter from Rapitest uses a flexible corded probe and large scale plate for easy reading. The incubation temperature should be 37 &… Utilizing proprietary surface technologies and 3 µm particle size, BioMix-300 phase is made of uniform, hydrophilic, and neutral nanometer thick films chemically bonded on the high purity and enhanced mechanical stability silica. Waters RapiGest SF, 1 mg, 5/pk Product Number: 186001861 RapiGest™ SF, is a innovative anionic surfactant that drastically improves in-solution protein digestion in terms of speed and peptide recovery. This part contains five, 1mg Oct 4, 2011 · Reduction / Alkylation (DTT, IAA and RapiGest or PPS) Prepare 0. The samples were then incubated on a shaker for 48 h at 4°C followed by heating at 80°C for 10 min. Flex and Focus custom panels, tissue The mass spectrometry-compatible surfactant RapiGest promotes the enzymatic digestion of proteins by facilitating their unfolding while retaining enzymatic activity. Bioz Stars score: 85/100, based on 10 PubMed citations. RapiGest SF, 10 mg, 1/pk Enjoy exclusive benefits including discounted pricing on orders by contacting our Sales Executives to open an account. We offer unique ranges of HPLC columns for bioanalytical characterization and testing, as well as process media for downstream process and purification of MAb, BsMAb, ADC, Proteins, AAV, VLP, DNA, RNA, and various biologic samples. TIP: Therapeutic proteins or mAbs with higher hydrophobic characteristic, might require a higher amount of the RapiGest SF. RapiGest SF is particularly valuable in proteomics workflows where efficient protein digestion and high peptide recovery are essential. rapigest (digestive tonic) offered by b&t pharmaceuticals is available with multiple payment options and easy delivery. 2. This part contains one, 50 mg vial Find out all of the information about the Waters Ges. 76Regular Price$2,743. 8). Contains information for both use and recommendations for how to adjust soil pH and/or choose the correct plants, based on your results Note: RapiGest SF hydrolyzes in acidic solutions (half life 8 min. 00/ea. The kit contains: GlycoWorks Deglycosylation Module: (1) 0. Digestion parameters RapiGest SF, is a patented anionic surfactant that drastically improves in-solution protein digestion in terms of speed and peptide recovery. Storage and stability also support its use to solubilize glycoproteins prior to deglycosylation with enzymes such as PNGase F (Publication in Buy low price rapigest (digestive tonic) in cherlapally, hyderabad. Above prices effective July 1, 2025 and are subject to change. com Waters RapiGest SF, 1 mg, 1/pk - 186001860 - RapiGest™ SF Surfactant is a reagent used to enhance enzymatic digestion of proteins. Make up Rapigest with 50 mM AmBic. * **How much does Rapigest SF Surfactant cost?** This is the big one. Recent investigations II. The optimum RapiGest concentration is 0. at pH 3). By helping solubilize proteins, RapiGest SF makes them more susceptible to enzymatic cleavage without significantly inhibiting enzyme activity and remains heat stable to support higher temperature digestions. High Quality proteomic research services, Protein Identification, Quantification and Sequencing, Highly Competitive Proteomics Cost and Mass Spectrometry Test Cost and Fast Turnaround Time. 25 mL/vial Glycoworks Rapid Buffer (1) 4 vials of 10 mg/vial RapiGest SF Surfactant This basic kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, and the GlycoWorks Clean-up Module (excludes PN 186007988). RapiGest consists of a hydrophilic head and a hydrophobic tail, which can be separated by acid hydrolysis. This part contains five, 1mg vials that contain enough material for 1-50 digestions. Myoglobin was solubilized in 0. We would like to show you a description here but the site won’t allow us. com UVISON. We evaluate a set of protocols for preparation of the secretome from murine Islets of Langerhans for bottom-up proteomic analysis. This part contains one Surfactant Rapigest, supplied by Waters Corporation, used in various techniques. This part contains one, 3 Using RapiGest SF, a complete tryptic digestion of horse myoglobin was achieved in minutes instead of hours (Figure 2A). Many hydrophobic proteins are resistant to proteolysis because their A total number of 229, 112, 138, and 203 proteins were identified for GnHCl, RapiGest TM , GnHCl and RapiGest TM and GnHCl followed by RapiGest TM respectively (Fig. 1 % Waters RapiGest SF used for in-solution work is RapiGest SF, produced by Waters. For example, in the textile industry, Rapigest is commonly used to improve the cleaning and lustration properties of fabrics. At Cenmed, your confidence and satisfaction are paramount. RapiGest SF, is a patented anionic surfactant that drastically improves in-solution protein digestion in terms of speed and peptide recovery. First introduced in 2002 as an enzyme-friendly surfactant to assist in-solution protein digestion, Rapi Gest SF is an innovative product created to improve protein solubility during sample preparation. III. INtroduction tion (Refs. This part contains one, 1mg vial that containing enough material for 1-10 digestions. Time estimates for data analysis charges are available upon request. J Mass Spectrom 2004 39 (2):202-7. 2C). 2% RapiGest per 100 μl protein mixture (1:1) to get a final concentration of RapiGest of 0. H product: solution reagent RapiGest SF. This part contains 4, 10 mg vials Oct 1, 2009 · We present a rapid and efficient in-solution enzymatic digestion protocol suitable for mass spectrometry-based absolute protein quantification techniques. RapiGest is a patented anionic surfactant that accelerates the in-solution production of peptides generated by proteases, such as trypsin, Asp-N, Glu-C, and Lys-C. RapiGest SF helps solubilize proteins, making them more susceptible to enzymatic cleavage without inhibiting enzyme activity. 10 working days or more Packaging Unit PCE $2,633. It contains materials to process 96 samples in a 4x24 format. **5. No reduction/alkylation reagents are used. RapiGest consists of a hydrophili RapiGest SF Surfactant Protocol Use the RapiGest SF Surfactant from RapiGest Surfactant from Waters to extract and solubilize hydrophobic proteins and improve in-solution enzymatic digestions of proteins. This enables use of lesser amounts of expensive endoproteases. RapiGest SF, 50 mg, 1/pk Enjoy exclusive benefits including discounted pricing on orders by contacting our Sales Executives to open an account. If Mass Spec analysis is required, the recommended 沃特世科技（上海）有限公司为您提供RapiGest SF蛋白酶解表面活性剂价格、货号、参数等信息，厂家客服随时为您服务，欢迎来电或留言咨询售前、售后问题。 RapiGest™ SF is an anionic surfactant for peptide digestion. Add RapiGest&trade, SF to your proteolytic digestion, (using trypsin or other enzymes) to enhance digestion speed and increase peptide recovery. The mechanism by which Compare Proteomics Services from leading suppliers on Biocompare. Free access to current and historic data for Bitcoin and thousands of altcoins. Acid-labile sufactant. First Aug 27, 2021 · The mass spectrometry-compatible surfactant RapiGest promotes the enzymatic digestion of proteins by facilitating their unfolding while retaining enzymatic activity. We guarantee the quality and reliability of our extensive range of clinical and laboratory supplies. Data analysis is charged per hour following initial data reporting. Add RapiGest SF to your proteolytic digestion, (using trypsin or other enzymes) to enhance digestion speed and increase peptide recovery. 2. 5% or 10%). This part contains five, 1mg High Quality proteomic research services, Protein Identification, Quantification and Sequencing, Highly Competitive Proteomics Cost and Mass Spectrometry Test Cost and Fast Turnaround Time. I am trying to extract protein from a tissue sample for liquid chromatography mass spectrometry but I am not sure which concentration of Rapigest to use (0. Contact Us Rapigest is an acid-labile detergent used in proteomics for the improvement of protein digestion. 1% to 0. 8 (w/v) (1 mg RapiGest per 500 μl 50 mM AMBIC pH 7. Aug 11, 2025 · The Tektronix 11402A Oscilloscope with 11A16, 11A33 and 11A32 Plugins are all in very good used condition and all are operational. Displaying accurate real-time pricing information about items in Old School RuneScape, powered by RuneLite users. The price isn’t fixed like a candy bar. The proprietary surface technologies allow the chemistry of thin film formation to be well controlled, which results in high column-to-column reproducibility. Reference: 1) Nomura E etc. Please note: Prices below are based on an a-la-carte menu of services to maximize flexibility in experimental designs and comply with auditing regulations. US Patent No: 8,580,533. For each comparison Using RapiGest SF, a complete tryptic digestion of horse myoglobin was achieved in minutes instead of hours (Figure 2A). ALS-1, also known as RapiGest SF, is an acid-Liable surfactant. Most analyses will require Shop Waters Corp RapiGest SF, 10 mg, 4/pk at Fishersci. 1% RapiGest™ in guanidine extraction buffer) was added to each sample before sonication (Section 2. RapiGest™ SF is Pricing For Core Service Support for Duke and Non-Duke Scientists Note: Scientific collaborations with academic, government, and non-profit laboratories outside Duke are welcome. 25 mL/vial Glycoworks Rapid Buffer (1) 3 vials of 3 mg/vial RapiGest SF Surfactant (1 Rates Prices are for each set of samples (6 – 16 Plex), effective as of September 1st, 2022 and are good through June 30, 2023. RapiGest SF, 200 mg, 1/pk. The incubation temperature should be 37 &… RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. RapiGest™ SF is available in a variety of formats. Othe We would like to show you a description here but the site won’t allow us. RapiGest™ SF, is a patented anionic surfactant that drastically improves in-solution protein digestion in terms of speed and peptide recovery. ZERO BIAS - scores, article reviews, protocol conditions and more The majority of mass spectrometry-based protein quantification studies uses peptide-centric analytical methods and thus strongly relies on efficient and unbiased protein digestion protocols for sample preparation. 2% RapiGest in 50 mM AMBIC pH 7. RapiGest SF is available in a variety of formats. RapiGest&trade, SF is available in a variety of formats. This patented anionic surfactant accelerates the in-solution production of peptides generated by proteases, such as Trypsin, Asp-N, Glu-C, Lys-C, and PNGase F. SF Protein Digestion Surfactant Waters patented* RapiGestTM SF (surfactant) radically enhances protein enzymatic digestions in terms of speed and percent recovery. 2-6) enzymatic digestions of proteins. It contains materials to process 24 samples in a 3x8 format. Of the protocols evaluated, a filter-aided sample preparation based approach using sodium dodecyl sulfate as a Jan 20, 2024 · Great prices on a wide variety of overstocks, close-outs, freight recovery & more, including deals on Cisco, Nortel, HP, Alcatel, NetApp, IBM, Sun, Symbol, Hypercom, Zebra, etc. 1%. "Acid-labile surfactant improves in-sodium dodecyl sulfate polyacrylamide gel protein digestion for matrix-assisted laser desorption/ionization mass spectrometric peptide mapping". RapiGest SF Surfactant is a reagent used to enhance enzymatic digestion of proteins, both in-gel and in-solution. Rapigest SF Surfactant FAQs: Cost and More** Let’s tackle those burning questions, especially the price tag. Top cryptocurrency prices and charts, listed by market capitalization. Many hydrophobic proteins are resistant to proteolysis because their RapiGest SF, is a patented anionic surfactant that drastically improves in-solution protein digestion in terms of speed and peptide recovery. This allows for removal of … ADDITIONAL INFORMATION The function of RapiGest SF is to denature a protein; it is not going to cause protein digestion. Contact Us RapiGest SF 是一种创新型阴离子表面活性剂，可大大提高蛋白质在溶液中酶解的速度和肽回收率。 它能避免使用其他常见表面活性剂时容易出现的高背景噪音问题，因此在质谱 (MS) 分析的蛋白质样品制备中尤其有用。 RapiGest SF 可以多种形式提供。 该部分包括 5 个 1 mg 的样品瓶，含有足够进行 1-50 次酶 In this application note, we provide an overview of the physical and chemical characteristics of Waters’ patented Rapi Gest SF and illustrate selected application areas. 6 days ago · Gasoline and diesel fuel prices released weekly. Feb 19, 2018 · RapiGest SF, 1 mg , 5/pk Manufacturer Waters Corporation Product code 186001861 Revision date 2021 August 02 Language English RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. It helps to reduce background noise often associated with other surfactants, improving the sensitivity and quality of MS analysis. Information and reports on Rapigest Sf 1mg Imports Under Sub Chapter 9027 along with detailed shipment data, import price, export price, monthly trends, major exporting countries countries, major importing countries and major ports. RapiGest SF 表面活性剂 RapiGest SF 表面活性剂是一种突破性的解决方案，旨在显著提高溶液内酶促蛋白质消化的效率和效果。 全球各地的实验室不断寻求简化消化协议和提高分析通量的方法，而 RapiGest SF 则是实现这些目标的工具。 6 days ago · Television's longest-running game show, featuring host Drew Carey, where audience members try to win cash and prizes. RapiGest SF, 10 mg, 1/pk Enhance in-gel and in-solution enzymatic digestion of proteins with the reagent RapiGest SF. Find many great new & used options and get the best deals for Waters RapiGest SF Surfactant 10 mg at the best online prices at eBay! Free shipping for many products! Amazon. This could be longer for very hydrophobic proteins. 00 Qty: 1 How it works Request your Quote Buy RapiGest SF, 186002123, from Waters Corporation India. I have this seller on my favored sellers list for their many high-quality items, great prices and excellent shipping. For current pricing, please reach out to Mandy Bittner for a formal quote. Jan 20, 2024 · Great prices on a wide variety of overstocks, close-outs, freight recovery & more, including deals on Cisco, Nortel, HP, Alcatel, NetApp, IBM, Sun, Symbol, Hypercom, Zebra, etc. Enable uncompromising speed in protein denaturation and enzymatic protein digestion applications with RapiZyme Trypsin, MS Grade reagent. 1. The logarithmic scale is properly calibrated to give the most accurate results. 1 %, 0. 035 mL/vial Rapid PNGaseF Enzyme (4) 0. This part contains 4, 10 mg vials RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. 1% of RapiGest SF in 50 mM ammonium bicarbonate buffer, pH 7. A quote will include the cost of sample preparation when these supplies are used. Contact us for details. RapiGest SF is an MS-compatible mild protein denaturant that helps solubilize and unfold proteins, making them more amenable to cleavage without denaturing or inhibiting common proteolytic enzymes like conventional denaturants. 1). GST/HST. Supplied as five 1 mg vials for 1 to 50 digestions. 9 and digested with trypsin (Promega) at 37 °C. m. RapiGest™ SF, is a innovative anionic surfactant that drastically improves in-solution protein digestion in terms of speed and peptide recovery. 1) and in-solu- I. How RapiGest Works Rapi Gest SF is a mild denaturant that solubilizes and unfolds the substrate protein, making it more amenable to cleavage. In addition to its price, the cost of Rapigest also depends on the application it is being used for. RapiGest™ SF is UChicago Proteomics Platform will purchase it and charge it at market prices to the customers. This part contains one, 10 We would like to show you a description here but the site won’t allow us. 25% - 0. (B) Schematic workflow of follow up experiment using the chaotropic agent GnHCl, the surfactant RapiGest™, a combination of GnHCl and RapiGest™, and a combination of GnHCl followed by RapiGest™ extraction on the insoluble pellet. This part contains one, 3 RapiGest SF is available in a variety of formats. US Patent No: 8,580,533 Enjoy exclusive benefits including discounted pricing on orders by contacting our Sales Executives to open an account. May 26, 2017 · All identified proteins in each method are can be found in Supporting Information Table 1. 50 Excl. This is an acid-cleavable detergent. RapiGest SF is an anionic surfactant that allows for better percent trypsin activity than when trypsin is used in the presence of some other denaturants. Many hydrophobic proteins are resistant to proteolysis because their The cost of Rapigest can range from $1 to several thousand dollars per ton. It is especially valuable in protein sample preparation for mass spectrometry (MS) analysis by avoiding high background noise often seen from other common surfactants. ORG provides you with fast loading charts of the current gold price per ounce, gram and kilogram in 160 major currencies. Introduction Contents RapiGest™ SF is a reagent used to enhance in-gel (Ref.